The basic principle of chromatography and the different types of chromatography. There are many ways to classify liquid column chromatography. Chromatography is a versatile method of separating many different kinds of chemical mixtures. Top 12 types of chromatographic techniques biochemistry. In this type of chromatography, the use of a resin the stationary solid phase is used to. There are many types of chromatography like hplc, gas chromatography, paper chromatography, thin layer chromatography, etc. The type of interaction between stationary phase, mobile phase, and. This video is an explanation about the principle of chromatography. In this lesson, learn the different types and uses of the technique. Different types of pumps are used in chromatography system, mostly based on the pressure level required to perform chromatography. Column chromatography is one of the most common methods of protein. Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The sample is here introduced in its entirety without heat.
Adsorption column chromatography adsorption chromatography is a technique of separation, in which the components of the mixture are adsorbed on the surface of the adsorbent. This book will provide a basic introduction to different types of liquid and gas chromatography. In this book contains more details about the applications of chromatography by. To fully understand the chromatographic techniques involved with this type of chemistry and the principles that underline. Chromatography is an analytic technique which is based on the separation of molecules of a sample over two phases. Partition column chromatography the stationary phase, as well as mobile phase, are liquid in partition chromatography. The basic principle is that components in a mixture have different tendencies to adsorb onto a surface or dissolve in a solvent. All of the instrumental chromatographic techniques are now used routinely by academic. These include column chromatography, high performance liquid chromatography hplc, gas chromatography, size exclusion chromatography, ion exchange chromatography etc. Chromatography the classification of chromatography. If this classification is based on the nature of the stationary phase and the separation process, three modes can be specified. The stationary phase is one which stays motionless and allows the sample to move over it.
The stationary phase is usually silica or aluminaa very polar layer of adsorbent on an inert, flat support. Chromatography is a method used by scientists for separating organic and inorganic. Chromatography definition, principle, types, applications. What are the different types of paper chromatography answers. Gas chromatography gc high pressure liquid chromatography hplc thin layer chromatography tlc. For details go through types of chromatography, but common technical requirements to all the types include. Commonly employed chromatography techniques include. These phases are called as the stationary and the mobile phase. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed paper chromatography or a layer of solid particles spread on a support such as a glass plate thin layer chromatography.
The basic principle of chromatography and the different. Column chromatography principle, procedure, applications. The following points highlight the top three types of chromatography techniques. This lecture explains about different types of chromatography in hindi. Chromatography is an important biophysical technique that enables the separation, identification, and purification of the.